Ret Proto-Oncogene antibody (Extracellular) (FITC)

Catalog No. ABIN7582071

This anti-Ret Proto-Oncogene antibody is a Rabbit Polyclonal antibody detecting Ret Proto-Oncogene in FACS and LCI. Suitable for Human.

Quick Overview for Ret Proto-Oncogene antibody (Extracellular) (FITC) (ABIN7582071)

Target: Ret Proto-Oncogene (RET)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ret Proto-Oncogene antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Ret Proto-Oncogene Antibody (FITC)

Binding Specificity: AA 541-553, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Ret receptor (extracellular) conjugated to the fluorescent dye FITC

Sequence: CEWRQGDGKG ITR

Specificity: Extracellular, N-terminus

Predicted Reactivity: Mouse - identical, rat - 12,13 amino acid residues identical

Characteristics: Anti-Ret Receptor (extracellular) Antibody (ABIN7043600, ABIN7044783 and ABIN7044784) is a highly specific antibody directed against an extracellular epitope of the human Ret receptor. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize Ret receptor from mouse, rat and human samples. Anti-Ret Receptor (extracellular)-FITC Antibody (ABIN7043600, ABIN7044783 and ABIN7044784-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize Ret receptor from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CEWRQGDGKGITR, corresponding to amino acid residues 541-553 of human Ret

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236725)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Ret Proto-Oncogene

Target: Ret Proto-Oncogene (RET)

Alternative Name: RET

Background: Proto-oncogene tyrosine-protein kinase receptor Ret, Proto-oncogene c-Ret, Cadherin family member 12, CDHF12, PTC, RET51,The RET proto-oncogene on human chromosome 10q11.2, encodes a receptor tyrosine kinase (RTK) activated by members of the glial cell line-derived neurotrophic factor (GDNF) ligand family (GDNF, neurturin, artemin, and persephin) in conjunction with a ligand-specific coreceptor (GFRα1-4)1. RET belongs to the cadherin superfamily, and it has been suggested that the ret gene is the result of a recombination between a gene encoding a tyrosine kinase receptor and a gene encoding an ancestral cadherin at an early stage of evolution2. The extracellular domain of RET comprises, as observed for other tyrosine kinase receptors, a cysteine-rich domain, but also four cadherin-like domains3.RET signaling is crucial for the development of the enteric nervous system. RET also regulates the development of sympathetic, parasympathetic, motor, and sensory neurons, and is necessary for the postnatal maintenance of dopaminergic neurons. Outside the nervous system, RET is crucial for development of the kidney and plays a key role in spermatogenesis4.RET has attracted considerable clinical interest because of the range of mutations found in diverse conditions that include Hirschsprung disease and a variety of cancers involving the thyroid gland. RET-related cancers with thyroid involvement include sporadic and familial medullary thyroid carcinoma (MTC), multiple endocrine neoplasia 2 (MEN2) syndromes MEN2A and MEN2B, and papillary thyroid carcinoma (PTC)5.

Gene ID: 5979

UniProt: P07949

Pathways: RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation